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moo man
moo man 發問於 Science & MathematicsBiology · 10 年前

Decolorizing Agent in Gram Stain?

Is it really important to use 95% alcohol or can I use 70% to 90% with similar to duplicate results?

3 個解答

相關度
  • saffronesque
    Lv 7
    10 年前
    最愛解答

    Decolorization will take longer to occur with a more dilute solution. If you have a good supply of gram positive and gram negative bacteria, you could do a series of slides and increase the length of exposure to the more dilute alcohol solution until you get good decolorization of the gram negative smears. Some procedures uses an acetone-alcohol solution in place of 95% ethanol.

    資料來源： Microbiology instructor
  • ?
    Lv 4
    4 年前

    Gram-unfavorable bacteria have peptidoglycan interior the outer layer of their cellular wall, and gram-effective bacteria have peptidoglycan of their cellular wall a number of layers down. The stain reacts with the peptidoglycan.

  • 匿名
    10 年前

    i always used isotropically

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